Publications
2024
Arp2/3-dependent actin assembly shapes endosomes and promotes intracellular trafficking in fission yeast
Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Cdc42 mobility and membrane flows regulate fission yeast cell shape and survival.
2023
Revised fission yeast gene and allele nomenclature guidelines for machine readability.
A focus on yeast mating: From pheromone signaling to cell-cell fusion.
2022
Condensation of the fusion focus by the intrinsically disordered region of the formin Fus1 is essential for cell-cell fusion.
mNG-tagged fusion proteins and nanobodies to visualize tropomyosins in yeast and mammalian cells.
Actin assembly requirements of the formin Fus1 to build the fusion focus.
2021
Going with the membrane flow: the impact of polarized secretion on bulk plasma membrane flows.
Ultrastructural plasma membrane asymmetries in tension and curvature promote yeast cell fusion.
Cell patterning by secretion-induced plasma membrane flows.
Direct and indirect regulation of Pom1 cell size pathway by the protein phosphatase 2C Ptc1.
Cell cycle-dependent and independent mating blocks ensure fungal zygote survival and ploidy maintenance.
2020
Activation of Cdc42 GTPase upon CRY2-Induced Cortical Recruitment Is Antagonized by GAPs in Fission Yeast.
Sterol biosensor reveals LAM-family Ltc1-dependent sterol flow to endosomes upon Arp2/3 inhibition.
DYRK kinase Pom1 drives F-BAR protein Cdc15 from the membrane to promote medial division.
Optogenetics reveals Cdc42 local activation by scaffold-mediated positive feedback and Ras GTPase.
A toolbox of stable integration vectors in the fission yeast Schizosaccharomyces pombe.
2019
Capping Protein Insulates Arp2/3-Assembled Actin Patches from Formins.
Quorum sensing with pheromones.
Molecular mechanisms of chemotropism and cell fusion in unicellular fungi.
Multi-phosphorylation reaction and clustering tune Pom1 gradient mid-cell levels according to cell size.
Yeast-to-hypha transition of Schizosaccharomyces japonicus in response to environmental stimuli.
2018
Differential GAP requirement for Cdc42-GTP polarization during proliferation and sexual reproduction.
Gamete fusion triggers bipartite transcription factor assembly to block re-fertilization.
Exploration and stabilization of Ras1 mating zone: A mechanism with positive and negative feedbacks.
Dynamic visits of cortical structures probe for cell size.
Inhibition of Ras activity coordinates cell fusion with cell-cell contact during yeast mating.
2017
Live Cell Imaging of the Schizosaccharomyces pombe Sexual Life Cycle.
A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Nuclear displacement and fluorescence recovery after photobleaching (FRAP) assays to study division site placement and cytokinesis in fission yeast.
2016
Role and organization of the actin cytoskeleton during cell-cell fusion.
Spatial focalization of pheromone/MAPK signaling triggers commitment to cell-cell fusion.
Local Pheromone Release from Dynamic Polarity Sites Underlies Cell-Cell Pairing during Yeast Mating.
Microscopy of Fission Yeast Sexual Lifecycle.
2015
Editorial overview: Eukaryotic microbes: models and beyond.
The DYRK-family kinase Pom1 phosphorylates the F-BAR protein Cdc15 to prevent division at cell poles.
Spontaneous cell polarization: Feedback control of Cdc42 GTPase breaks cellular symmetry.
PKA antagonizes CLASP-dependent microtubule stabilization to re-localize Pom1 and buffer cell size upon glucose limitation.
Pom1 gradient buffering through intermolecular auto-phosphorylation.
Casein kinase 1γ ensures monopolar growth polarity under incomplete DNA replication downstream of Cds1 and calcineurin in fission yeast.
Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based trafficking.
A formin-nucleated actin aster concentrates cell wall hydrolases for cell fusion in fission yeast.
2014
Pom1 regulates the assembly of Cdr2-Mid1 cortical nodes for robust spatial control of cytokinesis.
The novel proteins Rng8 and Rng9 regulate the myosin-V Myo51 during fission yeast cytokinesis.
The Tea4-PP1 landmark promotes local growth by dual Cdc42 GEF recruitment and GAP exclusion.
Yeasts as models in cell biology.
Cell polarization in budding and fission yeasts.
Distinct levels in Pom1 gradients limit Cdr2 activity and localization to time and position division.
2013
Mate and fuse: how yeast cells do it.
Cdc42 explores the cell periphery for mate selection in fission yeast.
2012
Cdc42 oscillations in yeasts.
Fission yeast: in shape to divide.
Myosin Vs organize actin cables in fission yeast.
Being at the right place at the right time.
Fission yeast Sec3 and Exo70 are transported on actin cables and localize the exocyst complex to cell poles.
2011
Shaping fission yeast cells by rerouting actin-based transport on microtubules.
Deconstructing the cell cycle.
A phosphorylation cycle shapes gradients of the DYRK family kinase Pom1 at the plasma membrane.
Actin cables and the exocyst form two independent morphogenesis pathways in the fission yeast.
2009
Geometric control of the cell cycle.
Pob1 participates in the Cdc42 regulation of fission yeast actin cytoskeleton.
Microtubule-dependent cell morphogenesis in the fission yeast.
Shaping fission yeast with microtubules.
Polar gradients of the DYRK-family kinase Pom1 couple cell length with the cell cycle.
LKB1 regulates polarity remodeling and adherens junction formation in the Drosophila eye.
2007
Regulation of the formin for3p by cdc42p and bud6p.
2006
The cell-end factor pom1p inhibits mid1p in specification of the cell division plane in fission yeast.
Dynamics of the formin for3p in actin cable assembly.
2005
New end take off: regulating cell polarity during the fission yeast cell cycle.
Cell Cycle,
2005 Aug;
4
(8): 1046-1049
Tea4p links microtubule plus ends with the formin for3p in the establishment of cell polarity.
Regulation of actin assembly by microtubules in fission yeast cell polarity.
Novartis Found Symp,
2005;
269
: 59-66; discussion 66-72, 223-30
2003
Cell polarity: a new mod(e) of anchoring.
The identification of novel genes required for Drosophila anteroposterior axis formation in a germline clone screen using GFP-Staufen.
A role for Drosophila LKB1 in anterior-posterior axis formation and epithelial polarity.
2001
Ku-deficient yeast strains exhibit alternative states of silencing competence.
A rapid method to map mutations in Drosophila.
2000
The dynamics of yeast telomeres and silencing proteins through the cell cycle.
1999
Relocalization of telomeric Ku and SIR proteins in response to DNA strand breaks in yeast.
1998
Mutation of yeast Ku genes disrupts the subnuclear organization of telomeres.
Targeting Sir proteins to sites of action: a general mechanism for regulated repression.