Guichard/Hamel

Publications

Oct
Preprint
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Pfister JA, Agostini L, Bournonville L., Sankaralingam P., Bell ZG., Hamel V., Guichard P., Biertumpfel C., Mizuno N,  O'Connell KF
BioRxiv - Octobe 2024 -Link
 
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Bournonville L., Laporte M.H., Borgers S.,  Guichard P.*,  Hamel V.*
BioRxiv - October 2024 -Link
 
 
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Mercey O.,  Gadadhar S.,  Magiera MM, Lebrun L.,  Kostic C., Moulin A.,  Arsenijevic Y.,  Janke C.*,  Guichard P.*,  Hamel V.*
BioRxiv - April 2024 -Link
 
Guguin J, Chen TY,  Besson A,  Cuinat S,  Bertiaux E, Boutaud L, Ardito N, Murguiondo MI, Cabet S,  Hamel V, Thomas S, Pain B,  Edery P,  Putoux A,  Tang TK,  Mazoyer S,  Delous M.
MedRxiv - April 2024 -Link
 
2024
 
Ganga AK, Sweeney LK,  Rubio-Ramos A, Bishop C.S., Hamel V,  Guichard P,  Breslow DK.
Current Biology - September 2024 - Link
 
Burdet V.*, Bournonville L.*, Das M., Wenger E., Delattre M., Steiner FA§, Guichard P.§, and Hamel V.§
microPublication Biology - April 2024 - Link 
 
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Mercey O.#, Mukherjee S. #, Guichard P.*, Hamel V.*
Current Opinion in Cell Biology - June 2024 -Link
 
 
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Laporte MH, Gambarotto D., Bertiaux E., Bournonville L., Louvel V., Nunes JM, Borgers S., Hamel V.*, Guichard P.* 
Cell - April 2024 -Link
 
Arsenijevic Y., Chang N., Mercey O., El Fersioui Y., Koskiniemi-Kuendig H., Joubert C., Rivolta C., Sharon D., Guichard P., Hamel V., Kostic C.
EMBO Molecular Medecine - March 2024 -Link
 
2023
 
Louvel V., Haase R., Mercey O., Laporte M.H., Soldati-Favre D., Hamel V.*, Guichard P.*
Nature communications -November 2023-  Link
 
Arslanhan M.D., Cengiz-Emek S., Odabas E., Steib E.,  Hamel V.,  Guichard P.,  Firat-Karalar E.N.
Journal of Cell Biology   - November 2023 - Link
 
 
Klena N., Maltinti G., Batman U., Pigino G. *, Guichard P.*, and Hamel V*
Bio-protocol September 2023 
 
<Review in french>
Laporte MH, Bertiaux E., Hamel V.*, Guichard P.*
médecine/sciences -  April 2023 - Link
 
Eloy T., Baudrier E., Laporte M., Hamel V., Guichard P. , Fortun D.
IEEE Transactions on Computational Imaging - April 2023 -Link 
 
 
Muroňová J., Kherraf ZE, Giordani E., Eckert S., Cazin C., Amiri-Yekta A. , lambert E., Chevalier G., Martinez G., Neirijnck Y., Kühne F., Wehrli L., Klena N., Hamel V.,  Escoffier J.,  Guichard P., Coutton C., Fourati Ben Mustapha S., Kharouf M., Zouari R., Thierry-Mieg N.,  Nef S., Geimer S., Loeuillet C.,  Ray P.,  Arnoult C.
eLIFE - April 2023- Link
 
Probing the sub-cellular mechanisms of LCA5-Leber Congenital Amaurosis and associated gene therapy with expansion microscopy
Faber S.* Mercey O.*, Junger K., Garanto A., Ueffing M.,  Collin R.W.J. , Boldt K., Guichard P.*,  Hamel, V.*, Roepman R.*
JCI Insight - April 2023 - Link 
 
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Hamel V.*, Guichard P. *, Hirono M.*
The Chlamydomonas Sourcebook. Volume 3: Cell Motility and Behavior. 3rd Edition.
Feb 2023Link
 
Deficiency of the minor spliceosome component U4atac snRNA secondarily results in ciliary defects in human and zebrafish. Khatri D.,  Putoux A.,  Cologne A., Kaltenbach S., Besson A., Bertiaux E., Guguin J., Fendler A.,  Dupont M.A., Benoit-Pilven C. , Grotto S., Ruaud L.,  Michot C: , Castelle M., Guët A., Guibaud L., Hamel V., Bordonné R.,  Leutenegger AL.,  Attié-Bitach T., Edery P.,  Mazoyer S.,  Delous M.
PNAS - Feb 2023. Link
 

2022

Hinterndorfer K.*, Laporte M.H.*, Mikus F.*,  Tafur Petrozzi L., Bourgoint C., Prouteau M.,  Dey G.*, Loewith R.*,  Guichard P.*,  Hamel V.*
Journal of Cell Science - Dec 2022. Link

 

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Laporte M.H.*, Bouhlel I.B.*, Bertiaux E., Giroud A., Borgers S., Azimzadeh J., Hervé S., Fachinetti D., Conduit P.T., Bornens M., Guichard P.*, Paoletti A.* and Hamel V.*. 
EMBO J. September 2022. Link

  

Le Borgne P.Laporte M.H.Greibill L.Lemullois M.Temagoult M.Rosnet O.Le Guennec M.Lignières L.Chevreux G.Koll F.Hamel V.Guichard P.Tassin AM. 

PLOS Biology - September 2022. Link

Van den Hoek H., Klena N., Alvarez G., Jordan MA, Schaffer M., Erdmann PS. Wan WN, Plitzko JN, Baumeister W., Pigino G.*, Hamel V.*, Guichard P.*, Engel BD.*
Science - July 2022. Link

 

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Mercey O., Kostic CBertiaux E.Giroud A.Sadian Y.Chang N.Arsenijevic Y.Guichard P. *Hamel V.*
PLOS Biology. June 2002. Link

 

8f54b094cfee0e6bdbef9ab87a4cbcf7_f375.png
 Laporte M.H., Klena N., Hamel V* and Guichard P*.
 Nature Methods. Jan 2022. Link open access
 
 

2021

Guichard P.*, Laporte MH, Hamel V.*
Semin Cell Dev Biol. 2021 Dec 9.
 
 
 
Hatzopoulos GN, Kükenshöner T, Banterle N, Favez T, Flückiger I, Hamel V, Andany S, Fantner GE, Hantschel O, Gönczy P.

 Nature communications. June 2021. Link open access 

 

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Bertiaux A., Balestra A., Bournonville L., Brochet M. *, Guichard P. *, Hamel V. * 

PLOS Biology. Link open access - BiorXiv - Prelights

  

f3332cf7a561f756f5a2970842b6d60c_f329.png Characterization of the Novel Mitochondrial Genome Segregation Factor TAP110 in Trypanosoma brucei.

Amodeo S.Kalichava A.Fradera-Sola A.Bertiaux-Lequoy E.Guichard P.Butter F.Ochsenreiter T.

                                    Journal of Cell Science. Link JCS BiorXiv - Prelights

 
Le Guennec M.†, Klena N.†, Hamel V.* and Guichard P.*
Current Opinion in Structural Biology. Review. February 2021
 
 
 
 2020

9d0257afef47539e64666549013b189a_f320.pngThe architecture of the centriole cartwheel-containing region revealed by cryo-electron tomography.

Klena N., Le Guennec M., Tassin AM, Van den Hoek H..Erdmann PS, Schaffer M., Geimer S., Aeschlimann G., Kovacik L., Goldie KN,  Stahlberg H. , Engel BD*, Hamel V.* and Guichard P.
EMBO J. September 2020 - Link open access- Link BiorXiv
 

81d7e6504618edb7032e169c7b24b315_f332.png

Novel features of centriole polarity and cartwheel stacking revealed by cryo-tomography

Nazarov S.Bezler A.Hatzopoulos G.,Nemčíková Villímová V.Demurtas D.Le Guennec M.Guichard P.Gönczy P.  

EMBO J. September 2020 - Link Open Access - Link BiorXiv.

 

 Steib E., Laporte MH, Gambarotto D., Olieric N., Zheng C., Borgers S., Olieric V., Le Guennec M., Koll F., Tassin AM, Steinmetz MO, Guichard P.*, Hamel V.*  

eLIFE, September 2020. Link open access - link BioRxiv

 

e25817f31b024fb913122dcbc366fc3e_f330.pngImproving the resolution of fluorescence nanoscopy using post-expansion labeling microscopy.

Hamel V.* and Guichard P.*

Methods in Cell Biology, August 2020. Link

 
Gambarotto D., Hamel V.*Guichard P.*
Methods in Cell Biology, July 2020. Link
 
 
 
72e60e9d6273c4738877ad5abb46cd02_f298.jpgMolecular resolution imaging by post-labeling expansion single molecular localization microscopy (Ex-SMLM).Zwettler F.U.Reinhard S.Gambarotto D., Bell T.D.MHamel V.*Guichard P.*Sauer M.* 
Nature communications., July 2020. Link open access - BiorXivPrelights - Press release
 
 
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Homogeneous multifocal excitation for high-throughput super-resolution imaging.Mahecic D., Gambarotto D., Douglass K.M., Fortun D., Banterle N., Le Guennec M., Ibrahim K., Gonczy P., Hamel V., Guichard P.., Manley S.
Nature Methods, June 2020. Link open access
 
 
 Tosetti N., Dos Santos Pacheco N., Maco B., Bournonville L., Hamel V., Guichard P., Soldati-Favre D. 
eLife. May 2020 Link open access
 
c95762fff2ba4db9f451a12f5f8df05a_f252.pngA helical inner scaffold provides a structural basis for centriole cohesion. Le Guennec M, Klena N., Gambarotto D., Laporte MH , Tassin AM, Van den Hoek H., Erdmann PS, Schaffer M., Kovacik L., Borgers S., Goldie KN, Stahlberg H., Bornens M., Azimzadeh J., Engel BD#, Hamel V.* and Guichard P.
Science Advances. Feb 2020 Link open access - Unige press release
 

2019

ceb77e383c200eab610e1644c4f4ea64_f243.png The RB530 antibody recognizes microtubules by immunofluorescence
Eloïse Bertiaux-Lequoy, Philippe Hammel, Virginie Hamel, Paul Guichard.
Antibody reports. Sept 2019. Link open access

 

 

9b9b582eeaea4df3eeba4e01e67fc584_f210.pngFlagellar microtubule doublet assembly in vitro reveals a regulatory role of tubulin C-terminal tails. M. Schmidt-Cernohorska, I. Zhernov, E. Steib, M. Le Guennec1, R. Achek, S. Borgers, D. Demurtas, L. Mouawad, Z. Lansky, V. Hamel* and P. Guichard*.
Science. Jan 2019. Link open access ReprintFull text 

 

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Imaging cellular ultrastructures using expansion microscopy (U-ExM).  Gambarotto D. ##, Zwettler F. U. ##, Schmidt-Cernohorska M. , Fortun D. , Borgers  S. , Heine J. , Schloetel J. G. , Reuss M. , Unser M. , Boyden E.S. , Sauer M. *, Hamel V. * and  Guichard P.*. 
Nature Methods. 2019. Link open access - Preprint - Prelights - Press release

 

2018

2b115074e5dc3e08d96d95b29a46c746_f223.pngIsolation, Imaging and fluorescent single particle reconstruction of Chlamydomonas centrioles. Klena1 N.§, Gambarotto D. §, Le Guennec M. , Borgers S., Guichard P.*, Hamel V.* 

JoVE. (139), e58109, doi:10.3791/58109. Open Access

 

67c530592064711ef21b934f40b0fe73_f196.pngReconstruction From Multiple Particles for 3D Isotropic Resolution in Fluorescence Microscopy. Fortun DGuichard PHamel VSorzano COSBanterle NGonczy PUnser M

IEEE Trans Med Imaging. 2018 May;37(5):1235-1246.  Open Access

 

82fbf44d9ffdf45b876bbd993ffd33d0_f197.pngSuper-resolution microscopy to decipher multi-molecular assemblies. Sieben CDouglass KMGuichard PManley S. 
Curr Opin Struct Biol. 2018 Apr;49:169-176. 

 

 

Bioessays. 2018 Apr;40(4):e1700241.  Open Access
 

 

2017
1c9d31769395b2a36933984fc5e88ca3_f199.pngTORC1 organized in inhibited domains (TOROIDs) regulate TORC1 activity. Prouteau MDesfosses ASieben CBourgoint CLydia Mozaffari NDemurtas DMitra AKGuichard PManley SLoewith R. 
Nature. 2017 Oct 12;550(7675):265-269. Unige press release

 

898954f5ad450de31e5ada264acca4c8_f200.pngIdentification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis. Hamel VSteib EHamelin RArmand FBorgers SFlückiger IBusso COlieric NSorzano COSSteinmetz MOGuichard P*Gönczy P.*  
Curr Biol. 2017 Aug 21;27(16):2486-2498.e6. Open Access

 

e827d3160bc25d9db72986238ae69fef_f201.pngCell-free reconstitution reveals centriole cartwheel assembly mechanisms. Guichard PHamel VLe Guennec MBanterle NIacovache INemčíková VFlückiger IGoldie KNStahlberg HLévy DZuber BGönczy P
Nat Commun. 2017 Mar 23;8:14813.  Open Access

 

2016 
a0eaea7d732a600b614be6147e4fab74_f202.pngThe Human Centriolar Protein CEP135 Contains a Two-Stranded Coiled-Coil Domain Critical for Microtubule Binding. Kraatz SGuichard PObbineni JMOlieric NHatzopoulos GNHilbert MSen IMissimer JGönczy PSteinmetz MO
Structure. 2016 Aug 2;24(8):1358-1371. Open Access

 

Klein HCGuichard PHamel VGönczy PSchwarz US
Sci Rep. 2016 Jun 8;6:27075.  Open Access
 
D Fortun, P Guichard, N Chu, M Unser 
IEEE Journal of Selected Topics in Signal Processing, 2016
 
 
 
58b34143a72b509126e17f323b14266b_f205.pngSAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture. Hilbert MNoga AFrey DHamel VGuichard PKraatz SHPfreundschuh MHosner SFlückiger IJaussi RWieser MMThieltges KMDeupi XMüller DJKammerer RAGönczy PHirono MSteinmetz MO
Nat Cell Biol. 2016 Apr;18(4):393-403. 

 

Guichard PGönczy P
Cilia. 2016 Mar 1;5:9. Review. Open Access

 

 

2015 

11b6b35df98dd662e9ac72917608d46f_f207.pngDirect visualization of dispersed lipid bicontinuous cubic phases by cryo-electron tomography. Demurtas DGuichard PMartiel IMezzenga RHébert CSagalowicz L
Nat Commun. 2015 Nov 17;6:8915. Open Access

  

 

Guichard PHamel VNeves AGönczy P
Methods Cell Biol. 2015;129:191-209. 

 

 

Gogendeau DGuichard PTassin AM
Methods Cell Biol. 2015;129:171-89. 

 

 2014
Hamel VGuichard PFournier MGuiet RFlückiger ISeitz AGönczy P
Biomed Opt Express. 2014 Aug 29;5(10):3326-36. Open Access 

 

 

 2013
9c8c948354f46dbf066ef70f537762b6_f209.pngNative architecture of the centriole proximal region reveals features underlying its 9-fold radial symmetry. Guichard PHachet VMajubu NNeves ADemurtas DOlieric NFluckiger IYamada AKihara KNishida YMoriya SSteinmetz MOHongoh YGönczy P
Curr Biol. 2013 Sep 9;23(17):1620-8. 

 

d0dc09d9f5d4fdd885c26bf941e75461_f213.pngHepatitis B subvirus particles display both a fluid bilayer membrane and a strong resistance to freeze drying: a study by solid-state NMR, light scattering, and cryo-electron microscopy/tomography. Grélard AGuichard PBonnafous PMarco SLambert OManin CRonzon FDufourc EJ

FASEB J. 2013 Oct;27(10):4316-26. 

 

b27a8d0b596be6b26151dc95739718da_f214.pngCaenorhabditis elegans centriolar protein SAS-6 forms a spiral that is consistent with imparting a ninefold symmetry. Hilbert MErat MCHachet VGuichard PBlank IDFlückiger ISlater LLowe EDHatzopoulos GNSteinmetz MOGönczy PVakonakis IProc Natl Acad Sci U S A. 2013 Jul 9;110(28):11373-8. 

 

Quiniou EGuichard PPerahia DMarco SMouawad L.
Structure. 2013 May 7;21(5):833-43. 

 

 

15efdc16dda4564cced4ab185364e3ca_f217.pngUse of red autofluorescence for monitoring prodiginine biosynthesis. Tenconi EGuichard PMotte PMatagne ARigali SJ Microbiol Methods. 2013 May;93(2):138-43. doi: 10.1016/j.mimet.2013.02.012. Epub 2013 Mar 18.

 

 

2012
d6685c0c8cec3fdcf0d9acf29d5752b1_f216.pngCartwheel architecture of Trichonympha basal body. Guichard PDesfosses AMaheshwari AHachet VDietrich CBrune AIshikawa TSachse CGönczy P
Science. 2012 Aug 3;337(6094):553. 

 

2011

4eb22c6f4156eda32aaddf5470250f99_f218.pngThree dimensional morphology of rabies virus studied by cryo-electron tomography. Guichard PKrell TChevalier MVaysse CAdam ORonzon FMarco SJ Struct Biol. 2011 Oct;176(1):32-40. 

 

 
2010
Guichard PChrétien DMarco STassin AM
EMBO J. 2010 May 5;29(9):1565-72. 

 

 

2b0e992e6f5b8d120cded45291eb89a3_f220.pngSelf-assembling SAS-6 multimer is a core centriole building block. Gopalakrishnan JGuichard PSmith AHSchwarz HAgard DAMarco SAvidor-Reiss T. 
J Biol Chem. 2010 Mar 19;285(12):8759-70. 
 
 
 2009

8fc1ad6ed4cb56bb090652aeffe82959_f221.pngInvolvement of HFq protein in the post-transcriptional regulation of E. coli bacterial cytoskeleton and cell division proteins. Zambrano NGuichard PPBi YCayrol BMarco SArluison VCell Cycle. 2009 Aug;8(15):2470-2. 

 

 

3bc0ba9d2bb07ade99097121cdfad03b_f222.pngVisualization of proteins in intact cells with a clonable tag for electron microscopy. Diestra EFontana JGuichard PMarco SRisco CJ Struct Biol. 2009 Mar;165(3):157-68.