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Uli K. Laemmli

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Uli K. Laemmli was a Full Professor at the Department of Molecular, University of Geneva from 1980-2005. 

1996 Louis-Jeantet Prize Winner

1988 Marcel Benoist Prize Winner

Pubmed

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2008

The anchor-away technique: rapid, conditional establishment of yeast mutant phenotypes.
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Mol Cell, ; 31 (6): 925-932

2006

Displacement of D1, HP1 and topoisomerase II from satellite heterochromatin by a specific polyamide.
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EMBO J, ; 25 (11): 2397-2408
Nup-PI: the nucleopore-promoter interaction of genes in yeast.
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Mol Cell, ; 21 (3): 379-391

2005

Chromosome structure: improved immunolabeling for electron microscopy.
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Chromosoma, ; 114 (5): 365-375

2004

ChIC and ChEC; genomic mapping of chromatin proteins.
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Mol Cell, ; 16 (1): 147-157

2003

A two-step scaffolding model for mitotic chromosome assembly.
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Dev Cell, ; 4 (4): 467-480
Structural and dynamic functions establish chromatin domains.
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Mol Cell, ; 11 (1): 237-248

2002

Chromatin boundaries in budding yeast: the nuclear pore connection.
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Cell, ; 109 (5): 551-562
Identification of a multicopy chromatin boundary element at the borders of silenced chromosomal domains.
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Chromosoma, ; 110 (8): 519-531
The AT-hook protein D1 is essential for Drosophila melanogaster development and is implicated in position-effect variegation.
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Mol Cell Biol, ; 22 (4): 1218-1232

2001

Specific targeting of insect and vertebrate telomeres with pyrrole and imidazole polyamides.
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EMBO J, ; 20 (12): 3218-3228

2000

Specific gain- and loss-of-function phenotypes induced by satellite-specific DNA-binding drugs fed to Drosophila melanogaster.
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Mol Cell, ; 6 (5): 1013-1024
Chromatin opening of DNA satellites by targeted sequence-specific drugs.
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Mol Cell, ; 6 (5): 999-1011

1999

Evidence for an antagonistic relationship between the boundary element-associated factor BEAF and the transcription factor DREF.
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Chromosoma, ; 108 (6): 375-383
Nuclear import of RPA in Xenopus egg extracts requires a novel protein XRIPalpha but not importin alpha.
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EMBO J, ; 18 (15): 4348-4358

1998

Identification of a class of chromatin boundary elements.
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Mol Cell Biol, ; 18 (12): 7478-7486
Facilitation of chromatin dynamics by SARs.
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Curr Opin Genet Dev, ; 8 (5): 519-525
In vivo analysis of scaffold-associated regions in Drosophila: a synthetic high-affinity SAR binding protein suppresses position effect variegation.
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EMBO J, ; 17 (7): 2079-2085

1997

The scs' boundary element: characterization of boundary element-associated factors.
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Mol Cell Biol, ; 17 (2): 999-1009

1996

A nuclear traffic jam: unraveling multicomponent machines and compartments.
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Curr Opin Cell Biol, ; 8 (3): 299-302
A nuclear traffic jam - unraveling multicomponent machines and compartments.
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Curr Opin Cell Biol, ; 8 (3): 299-302

1995

SARs are cis DNA elements of chromosome dynamics: synthesis of a SAR repressor protein.
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Cell, ; 83 (7): 1137-1148
Visualization of chromosomal domains with boundary element-associated factor BEAF-32.
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Cell, ; 81 (6): 879-889

1994

SARs stimulate but do not confer position independent gene expression.
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Nucleic Acids Res, ; 22 (21): 4386-4394
Study of the cell cycle-dependent assembly of the DNA pre-replication centres in Xenopus egg extracts.
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EMBO J, ; 13 (17): 4153-4164
Metaphase chromosome structure: bands arise from a differential folding path of the highly AT-rich scaffold.
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Cell, ; 76 (4): 609-622

1993

A simple method to renature DNA-binding proteins separated by SDS-polyacrylamide gel electrophoresis.
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Nucleic Acids Res, ; 21 (25): 6040-6041
SAR-dependent mobilization of histone H1 by HMG-I/Y in vitro: HMG-I/Y is enriched in H1-depleted chromatin.
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EMBO J, ; 12 (8): 3237-3247
A model for chromatin opening: stimulation of topoisomerase II and restriction enzyme cleavage of chromatin by distamycin.
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EMBO J, ; 12 (1): 115-126
From the chromosomal loops and the scaffold to the classic bands of metaphase chromosomes.
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Cold Spring Harb Symp Quant Biol, ; 58 : 755-765

1992

Identification of nuclear pre-replication centers poised for DNA synthesis in Xenopus egg extracts: immunolocalization study of replication protein A.
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J Cell Biol, ; 119 (1): 1-15
Scaffold-associated regions: cis-acting determinants of chromatin structural loops and functional domains.
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Curr Opin Genet Dev, ; 2 (2): 275-285
In vivo topoisomerase II cleavage of the Drosophila histone and satellite III repeats: DNA sequence and structural characteristics.
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EMBO J, ; 11 (2): 705-716

1991

Specific complex of human immunodeficiency virus type 1 rev and nucleolar B23 proteins: dissociation by the Rev response element.
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Mol Cell Biol, ; 11 (5): 2567-2575
Chromosome assembly in vitro: topoisomerase II is required for condensation.
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Cell, ; 64 (1): 137-148

1989

Preferential, cooperative binding of DNA topoisomerase II to scaffold-associated regions.
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EMBO J, ; 8 (13): 3997-4006
Highly preferential nucleation of histone H1 assembly on scaffold-associated regions.
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J Mol Biol, ; 210 (3): 573-585
Specific inhibition of DNA binding to nuclear scaffolds and histone H1 by distamycin. The role of oligo(dA).oligo(dT) tracts.
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J Mol Biol, ; 210 (3): 587-599

1988

The metaphase scaffold is helically folded: sister chromatids have predominantly opposite helical handedness.
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Cell, ; 55 (6): 937-944
Interaction of DNA with nuclear scaffolds in vitro.
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J Mol Biol, ; 200 (1): 111-125
Scaffold attachment of DNA loops in metaphase chromosomes.
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J Mol Biol, ; 200 (1): 101-109

1987

Relation of chromosome structure and gene expression.
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Philos Trans R Soc Lond B Biol Sci, ; 317 (1187): 563-574
Improved methods for the isolation of individual and clustered mitotic chromosomes.
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Exp Cell Res, ; 173 (1): 85-98

1986

Cohabitation of scaffold binding regions with upstream/enhancer elements of three developmentally regulated genes of D. melanogaster.
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Cell, ; 46 (4): 521-530
Genes and loops in 320,000 base-pairs of the Drosophila melanogaster chromosome.
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J Mol Biol, ; 190 (2): 255-258
Metaphase chromosome structure. Involvement of topoisomerase II.
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J Mol Biol, ; 188 (4): 613-629
The organisation of chromatin loops: characterization of a scaffold attachment site.
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EMBO J, ; 5 (3): 511-518

1984

Immunization in vitro and production of monoclonal antibodies specific to insoluble and weakly immunogenic proteins.
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Proc Natl Acad Sci U S A, ; 81 (24): 7897-7901
Mapping nucleolar proteins with monoclonal antibodies.
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J Cell Biol, ; 99 (6): 1981-1988
Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffold.
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Cell, ; 39 (1): 223-232
Interphase nuclear matrix and metaphase scaffolding structures.
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J Cell Sci Suppl, ; 1 : 103-122
Silver staining the chromosome scaffold.
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Chromosoma, ; 89 (3): 186-192

1983

Architecture of metaphase chromosomes and chromosome scaffolds.
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J Cell Biol, ; 96 (1): 84-93

1982

Higher order metaphase chromosome structure: evidence for metalloprotein interactions.
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Cell, ; 29 (1): 171-181
Evidence for two levels of DNA folding in histone-depleted HeLa interphase nuclei.
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J Mol Biol, ; 156 (2): 309-324
Non-histone proteins and long-range organization of HeLa interphase DNA.
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J Mol Biol, ; 156 (2): 325-344

1980

Cell cycle changes in Physarum polycephalum histone H1 phosphate: relationship to deoxyribonucleic acid binding and chromosome condensation.
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Biochemistry, ; 19 (10): 2240-2246
The detection of DNA-binding proteins by protein blotting.
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Nucleic Acids Res, ; 8 (1): 1-20

1979

Metaphase chromosome structure: evidence for a radial loop model.
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Cell, ; 17 (4): 849-858
Maturation of the head of bacteriophage T4: 9-aminoacridine blocks a late step in DNA packaging.
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Virology, ; 92 (1): 219-229

1978

Levels of organization of the DNA in eucaryotic chromosomes.
Pharmacol Rev, ; 30 (4): 469-476
Metaphase chromosome structure: the role of nonhistone proteins.
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Cold Spring Harb Symp Quant Biol, ; 42 Pt 1 : 351-360

1977

Isolation of a protein scaffold from mitotic HeLa cell chromosomes.
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Proc Natl Acad Sci U S A, ; 74 (11): 4937-4941
Role of nonhistone proteins in metaphase chromosome structure.
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Cell, ; 12 (3): 805-816
The structure of histone-depleted metaphase chromosomes.
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Cell, ; 12 (3): 817-828
Morphogenetic core of the bacteriophage T4 head. Structure of the core in polyheads.
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J Mol Biol, ; 111 (4): 459-485
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
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J Biol Chem, ; 252 (3): 1102-1106

1976

Studies on the maturation of the head of bacteriophage T4.
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Philos Trans R Soc Lond B Biol Sci, ; 276 (943): 15-28
Head length determination in bacteriophage T4: the role of the core protein P22.
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J Mol Biol, ; 103 (1): 155-174
Correlation between structural transformation and cleavage of the major head protein of T4 bacteriophage.
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Cell, ; 7 (2): 191-203

1975

Characterization of DNA condensates induced by poly(ethylene oxide) and polylysine.
Proc Natl Acad Sci U S A, ; 72 (11): 4288-4292

1974

Maturation of the head of bacteriophage T4. IV. The proteins of the core of the tubular polyheads and in vitro cleavage of the head proteins.
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Virology, ; 62 (2): 483-499
Maturation of the head of bacteriophage T4. III. DNA packaging into preformed heads.
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J Mol Biol, ; 88 (4): 749-765
Maturation of the head of bacteriophage T4. V. A possible DNA packaging mechanism: in vitro cleavage of the head proteins and the structure of the core of the polyhead.
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J Supramol Struct, ; 2 (2-4): 276-301

1973

Maturation of the head of bacteriophage T4. II. Head-related, aberrant tau-particles.
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J Mol Biol, ; 80 (4): 601-611
Maturation of the head of bacteriophage T4. I. DNA packaging events.
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J Mol Biol, ; 80 (4): 575-599
Bacteriophage T4 tail assembly: structural proteins and their genetic identification.
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J Mol Biol, ; 75 (2): 315-337

1971

Polypeptides of the tail fibres of bacteriophage T4.
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J Mol Biol, ; 62 (3): 465-477

1970

Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature, ; 227 (5259): 680-685
Form-determining function of the genes required for the assembly of the head of bacteriophage T4.
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J Mol Biol, ; 49 (1): 99-113
A factor preventing the major head protein of bacteriophage T4 from random aggregation.
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J Mol Biol, ; 47 (1): 69-85

1968

Studies on the morphopoiesis of the head of phage T-even. V. The formation of polyheads.
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Mol Gen Genet, ; 101 (4): 333-345

Former groups