Thanos Halazonetis
Thanos Halazonetis was a Full Professor at the Department of Molecular Biology and Molecular and Cell Biology, University of Geneva, from 2006-2025.
The laboratory of Thanos Halazonetis has moved to the Department of Visceral Surgery and Medicine of the Hospital of the University of Bern, Bern, Switzerland
2024
In vivo DNA replication dynamics unveil aging-dependent replication stress.
RECQL4 is not critical for firing of human DNA replication origins.
Transcription-replication conflicts underlie sensitivity to PARP inhibitors.
Homozygous substitution of threonine 191 by proline in polymerase η causes Xeroderma pigmentosum variant.
2023
High-resolution mapping of mitotic DNA synthesis under conditions of replication stress in cultured cells.
2022
RAD51 protects human cells from transcription-replication conflicts.
Mitotic DNA synthesis is caused by transcription-replication conflicts in BRCA2-deficient cells.
Non-covalent SARS-CoV-2 M(pro) inhibitors developed from in silico screen hits.
Beating cancer one carbon at a time.
2021
Delayed DNA break repair for genome stability.
A transcription-based mechanism for oncogenic β-catenin-induced lethality in BRCA1/2-deficient cells.
Genomic Instability Profiles at the Single Cell Level in Mouse Colorectal Cancers of Defined Genotypes.
A multi-pronged approach targeting SARS-CoV-2 proteins using ultra-large virtual screening.
A method to sequence genomic sites of mitotic DNA synthesis in mammalian cells.
2020
High-resolution mapping of mitotic DNA synthesis regions and common fragile sites in the human genome through direct sequencing.
Draining the FEN1s for cancer therapy.
2019
Remodeling Collapsed DNA Replication Forks for Cancer Development.
Monitoring early S-phase origin firing and replication fork movement by sequencing nascent DNA from synchronized cells.
Monitoring early S-phase origin firing and replication fork movement by sequencing nascent DNA from synchronized cells.
2018
Intragenic origins due to short G1 phases underlie oncogene-induced DNA replication stress.
2017
Enhanced Rate of Acquisition of Point Mutations in Mouse Intestinal Adenomas Compared to Normal Tissue.
The role of SMARCAL1 in replication fork stability and telomere maintenance.
Increased Cell Proliferation and Gene Expression of Genes Related to Bone Remodeling, Cell Adhesion and Collagen Metabolism in the Periodontal Ligament of Unopposed Molars in Growing Rats.
A Model to Investigate Single-Strand DNA Responses in G1 Human Cells via a Telomere-Targeted, Nuclease-Deficient CRISPR-Cas9 System.
Impaired liver regeneration in aged mice can be rescued by silencing Hippo core kinases MST1 and MST2.
2016
Mammalian RAD52 Functions in Break-Induced Replication Repair of Collapsed DNA Replication Forks.
Alternative lengthening of human telomeres is a conservative DNA replication process with features of break-induced replication.
POLD3 Is Haploinsufficient for DNA Replication in Mice.
R-spondin 1 and noggin facilitate expansion of resident stem cells from non-damaged gallbladders.
2015
DNA replication stress as a hallmark of cancer.
DNA replication stress as an Achilles' heel of cancer.
Ubiquitin-H2AX fusions render 53BP1 recruitment to DNA damage sites independent of RNF8 or RNF168.
2014
Conservative DNA replication.
Reversal of the DNA-binding-induced loop L1 conformational switch in an engineered human p53 protein.
PARP inhibitors and IR join forces to strike glioblastoma-initiating cells.
Break-induced replication repair of damaged forks induces genomic duplications in human cells.
2013
A high resolution genomic portrait of bladder cancer: correlation between genomic aberrations and the DNA damage response.
Functional interplay between the DNA-damage-response kinase ATM and ARF tumour suppressor protein in human cancer.
Ultraviolet-B-mediated induction of protein-protein interactions in mammalian cells.
2012
A single-nucleotide substitution mutator phenotype revealed by exome sequencing of human colon adenomas.
The helicase domain and C-terminus of human RecQL4 facilitate replication elongation on DNA templates damaged by ionizing radiation.
Structural basis of transcriptional gene silencing mediated by Arabidopsis MOM1.
2011
Exome sequencing identifies recurrent somatic MAP2K1 and MAP2K2 mutations in melanoma.
Crystal structure of a multidomain human p53 tetramer bound to the natural CDKN1A (p21) p53-response element.
Studies of genomic copy number changes in human cancers reveal signatures of DNA replication stress.
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity.
Genomic instability in induced stem cells.
SAHF, to senesce or not to senesce?
Cell Cycle,
2011 Mar 1;
10
(5): 738-739
2010
Oncogene-induced senescence: the bright and dark side of the response.
TopBP1 functions with 53BP1 in the G1 DNA damage checkpoint.
Expression, crystallization and preliminary X-ray diffraction analysis of the CMM2 region of the Arabidopsis thaliana Morpheus' molecule 1 protein.
Acta Crystallogr Sect F Struct Biol Cryst Commun,
2010 Aug 1;
66
(Pt 8): 916-918
DNA polymerases nu and theta are required for efficient immunoglobulin V gene diversification in chicken.
14-3-3 binding sites in the snail protein are essential for snail-mediated transcriptional repression and epithelial-mesenchymal differentiation.
Genomic instability--an evolving hallmark of cancer.
2009
Emerging common themes in regulation of PIKKs and PI3Ks.
Cyclic olefin homopolymer-based microfluidics for protein crystallization and in situ X-ray diffraction.
Microcollimator for micrometer-wide stripe irradiation of cells using 20-30 keV X rays.
Modulation of the E2F1-driven cancer cell fate by the DNA damage response machinery and potential novel E2F1 targets in osteosarcomas.
An oligomerized 53BP1 tudor domain suffices for recognition of DNA double-strand breaks.
2008
An oncogene-induced DNA damage model for cancer development.
2007
Defective in mitotic arrest 1/ring finger 8 is a checkpoint protein that antagonizes the human mitotic exit network.
Deregulated overexpression of hCdt1 and hCdc6 promotes malignant behavior.
Phosphorylation of ATR-interacting protein on Ser239 mediates an interaction with breast-ovarian cancer susceptibility 1 and checkpoint function.
Evaluation of claspin as a proliferation marker in human cancer and normal tissues.
2006
DNA damage signaling recruits the RNA polymerase II binding protein Che-1 to the p53 promoter.
Oncogene-induced senescence is part of the tumorigenesis barrier imposed by DNA damage checkpoints.
SCFbetaTrCP-mediated degradation of Claspin regulates recovery from the DNA replication checkpoint response.
Cellular responses to DNA damage: current state of the field and review of the 52nd Benzon Symposium.
2005
Valosin-containing protein phosphorylation at Ser784 in response to DNA damage.
ATM signaling and 53BP1.
Human LATS1 is a mitotic exit network kinase.
Nbs1 moving up in the world.
Activation of the DNA damage checkpoint and genomic instability in human precancerous lesions.
The CHFR mitotic checkpoint protein delays cell cycle progression by excluding Cyclin B1 from the nucleus.
2004
Methylated lysine 79 of histone H3 targets 53BP1 to DNA double-strand breaks.
Defective apoptosis and B-cell lymphomas in mice with p53 point mutation at Ser 23.
Functional interaction between BLM helicase and 53BP1 in a Chk1-mediated pathway during S-phase arrest.
53BP1, an activator of ATM in response to DNA damage.
Constitutively active DNA damage checkpoint pathways as the driving force for the high frequency of p53 mutations in human cancer.
Structural differences in the DNA binding domains of human p53 and its C. elegans ortholog Cep-1.
p53 and stress in the ER.
2003
53BP1 and NFBD1/MDC1-Nbs1 function in parallel interacting pathways activating ataxia-telangiectasia mutated (ATM) in response to DNA damage.
Cancer Res,
2003 Dec 15;
63
(24): 8586-8591
Inactivating mutations targeting the chfr mitotic checkpoint gene in human lung cancer.
Cancer Res,
2003 Nov 1;
63
(21): 7185-7189
The Chfr mitotic checkpoint protein functions with Ubc13-Mms2 to form Lys63-linked polyubiquitin chains.
Frequent hypermethylation of the 5' CpG island of the mitotic stress checkpoint gene Chfr in colorectal and non-small cell lung cancer.
Carcinogenesis,
2003 Jan;
24
(1): 47-51
2002
53BP1 functions in an ATM-dependent checkpoint pathway that is constitutively activated in human cancer.
Chk2 leaves the PML depot.
Crystal structure of the FHA domain of the Chfr mitotic checkpoint protein and its complex with tungstate.
Structure,
2002 Jul;
10
(7): 891-899
2001
Acetylation of p53 activates transcription through recruitment of coactivators/histone acetyltransferases.
Mol Cell,
2001 Dec;
8
(6): 1243-1254
Substitutions that compromise the ionizing radiation-induced association of p53 with 14-3-3 proteins also compromise the ability of p53 to induce cell cycle arrest.
Cancer Res,
2001 Oct 1;
61
(19): 7030-7033
2000
p53 binding protein 1 (53BP1) is an early participant in the cellular response to DNA double-strand breaks.
J Cell Biol,
2000 Dec 25;
151
(7): 1381-1390
A serine 37 mutation associated with two missense mutations at highly conserved regions of p53 affect pro-apoptotic genes expression in a T-lymphoblastoid drug resistant cell line.
Aberrant regulation and function of wild-type p53 in radioresistant melanoma cells.
Cell Growth Differ,
2000 Sep;
11
(9): 467-474
Chfr defines a mitotic stress checkpoint that delays entry into metaphase.
Chk2/hCds1 functions as a DNA damage checkpoint in G(1) by stabilizing p53.
Genes Dev,
2000 Feb 1;
14
(3): 278-288
The DNA damage checkpoint and human cancer.
Cold Spring Harb Symp Quant Biol,
2000;
65
: 489-498
1999
Phosphorylation of Ser-20 mediates stabilization of human p53 in response to DNA damage.
Proc Natl Acad Sci U S A,
1999 Nov 23;
96
(24): 13777-13782
Many faces of ATM: eighth international workshop on ataxia-telangiectasia.
Biochim Biophys Acta,
1999 Oct 29;
1424
(2-3): R45-R55
Change in oligomerization specificity of the p53 tetramerization domain by hydrophobic amino acid substitutions.
Involvement of a p53-dependent pathway in rubella virus-induced apoptosis.
p53 sites acetylated in vitro by PCAF and p300 are acetylated in vivo in response to DNA damage.
Mol Cell Biol,
1999 Feb;
19
(2): 1202-1209
Modulation of wild-type p53 activity by mutant p53 R273H depends on the p53 responsive element (p53RE). A comparative study between the p53REs of the MDM2, WAFI/Cip1 and Bax genes in the lung cancer environment. WAFI/Cip1 = WAF1/Cip1.
Anticancer Res,
1999 Jan-Feb;
19
(1A): 579-587
1998
Alterations of the p16-pRb pathway and the chromosome locus 9p21-22 in non-small-cell lung carcinomas: relationship with p53 and MDM2 protein expression.
ATM-dependent activation of p53 involves dephosphorylation and association with 14-3-3 proteins.
Identification of an additional negative regulatory region for p53 sequence-specific DNA binding.
Proc Natl Acad Sci U S A,
1998 May 26;
95
(11): 6079-6084
Effects of p53 mutants derived from lung carcinomas on the p53-responsive element (p53RE) of the MDM2 gene.
Br J Cancer,
1998;
77
(3): 374-384
1997
Hydrophobic side-chain size is a determinant of the three-dimensional structure of the p53 oligomerization domain.
CREB-binding protein and p300/CBP-associated factor are transcriptional coactivators of the p53 tumor suppressor protein.
Cancer Res,
1997 Sep 1;
57
(17): 3693-3696
Two tandem and independent sub-activation domains in the amino terminus of p53 require the adaptor complex for activity.
1996
Structure-based rescue of common tumor-derived p53 mutants.
Nat Med,
1996 Oct;
2
(10): 1143-1146
An engineered four-stranded coiled coil substitutes for the tetramerization domain of wild-type p53 and alleviates transdominant inhibition by tumor-derived p53 mutants.
Cancer Res,
1996 Jan 1;
56
(1): 158-163
1995
Binding of wild-type and mutant forms of p53 protein from human tumors to a specific DNA-sequence of the first intron of the h-ras oncogene.
Int J Oncol,
1995 Nov;
7
(5): 1035-1041
Specific recognition of a transcriptional element within the human h-ras protooncogene by the p53 tumor-suppressor.
Int J Oncol,
1995 Nov;
7
(5): 1029-1034
The dihedral symmetry of the p53 tetramerization domain mandates a conformational switch upon DNA binding.
EMBO J,
1995 Feb 1;
14
(3): 512-519
1993
Conformational shifts propagate from the oligomerization domain of p53 to its tetrameric DNA binding domain and restore DNA binding to select p53 mutants.
EMBO J,
1993 Dec 15;
12
(13): 5057-5064
Wild-type p53 adopts a 'mutant'-like conformation when bound to DNA.
EMBO J,
1993 Mar;
12
(3): 1021-1028
Both the helix-loop-helix and the leucine zipper motifs of c-Myc contribute to its dimerization specificity with Max.
Oncogene,
1993 Jan;
8
(1): 125-132
1992
An enhancer "core" DNA-binding and transcriptional activity is induced upon transformation of rat embryo fibroblasts.
Anticancer Res,
1992 Mar-Apr;
12
(2): 285-291
Predicted structural similarities of the DNA binding domains of c-Myc and endonuclease Eco RI.
Science,
1992 Jan 24;
255
(5043): 464-466
1991
Proximal regulatory domains of rat atrial natriuretic factor gene.
Circulation,
1991 Sep;
84
(3): 1256-1265
Familial holoprosencephaly associated with a translocation breakpoint at chromosomal position 7q36.
Determination of the c-MYC DNA-binding site.
Proc Natl Acad Sci U S A,
1991 Jul 15;
88
(14): 6162-6166
1990
Drosophila homolog of the mammalian jun oncogene is expressed during embryonic development and activates transcription in mammalian cells.
Proc Natl Acad Sci U S A,
1990 Aug;
87
(16): 6281-6285
1989
Relationship of clinical parameters to attachment loss in subsets of subjects with destructive periodontal diseases.
J Clin Periodontol,
1989 Oct;
16
(9): 563-568
1988
c-Jun dimerizes with itself and with c-Fos, forming complexes of different DNA binding affinities.
Cell,
1988 Dec 2;
55
(5): 917-924
Retinoic acid increases the sensitivity of the rat embryo fibroblast transformation assay.
Mol Cell Biol,
1988 Apr;
8
(4): 1845-1848
1987
Evolutionarily conserved regions of the human c-myc protein can be uncoupled from transforming activity.
Proc Natl Acad Sci U S A,
1987 Jan;
84
(1): 170-173
1986
Posterior capsulitis in a 9-year-old girl.
J Pedod,
1986 Winter;
10
(2): 176-182
1985
Clinical medicine for dental students.
J Dent Educ,
1985 Dec;
49
(12): 809-813
Pocket formation 3 years after comprehensive periodontal therapy. A retrospective study.