Emmanuel Levy

Publications

2025

Rapid and sensitive protein complex alignment with Foldseek-Multimer.
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Nat Methods, ; 22 (3): 469-472
Structural determinants of co-translational protein complex assembly.
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Cell, ; 188 (3): 764-777.e22

2024

Mutational biases favor complexity increases in protein interaction networks after gene duplication.
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Mol Syst Biol, ; 20 (5): 549-572
An atlas of protein homo-oligomerization across domains of life.
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Cell, ; 187 (4): 999-1010.e15

2023

CC(+) : A searchable database of validated coiled coils in PDB structures and AlphaFold2 models.
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Protein Sci, ; 32 (11): e4789
Mesoscale molecular assembly is favored by the active, crowded cytoplasm.
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bioRxiv, ;
Discriminating physiological from non-physiological interfaces in structures of protein complexes: A community-wide study.
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Proteomics, ; 23 (17): e2200323

2022

Molecular and environmental determinants of biomolecular condensate formation.
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Nat Chem Biol, ; 18 (12): 1319-1329
How gene duplication diversifies the landscape of protein oligomeric state and function.
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Curr Opin Genet Dev, ; 76 : 101966
Affinity and Valence Impact the Extent and Symmetry of Phase Separation of Multivalent Proteins.
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Phys Rev Lett, ; 129 (12): 128102
A unified statistical potential reveals that amino acid stickiness governs nonspecific recruitment of client proteins into condensates.
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Protein Sci, ; 31 (7): e4361
Synthetic condensate size correlates with yeast replicative cell age.
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MicroPubl Biol, ; 2022
The modular cell gets connected.
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Science, ; 375 (6585): 1093-1094
Mutant libraries reveal negative design shielding proteins from supramolecular self-assembly and relocalization in cells.
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Proc Natl Acad Sci U S A, ; 119 (5)
PDBe-KB: collaboratively defining the biological context of structural data.
Nucleic Acids Res, ; 50 (D1): D534-D542
QSalignWeb: A Server to Predict and Analyze Protein Quaternary Structure.
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Front Mol Biosci, ; 8 : 787510

2021

PDB-wide identification of physiological hetero-oligomeric assemblies based on conserved quaternary structure geometry.
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Structure, ; 29 (11): 1303-1311.e3
Altered Protein Abundance and Localization Inferred from Sites of Alternative Modification by Ubiquitin and SUMO.
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J Mol Biol, ; 433 (21): 167219
Abundance Imparts Evolutionary Constraints of Similar Magnitude on the Buried, Surface, and Disordered Regions of Proteins.
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Front Mol Biosci, ; 8 : 626729
"Structuromics": another step toward a holistic view of the cell.
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Cell, ; 184 (2): 301-303

2020

Not Going with the Flow: How Cells Adapt Internal Physics.
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Cell, ; 183 (6): 1462-1463
Designer protein assemblies with tunable phase diagrams in living cells.
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Nat Chem Biol, ; 16 (9): 939-945
Proteomic analysis reveals the direct recruitment of intrinsically disordered regions to stress granules in S. cerevisiae.
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J Cell Sci, ; 133 (13)
PDBe-KB: a community-driven resource for structural and functional annotations.
Nucleic Acids Res, ; 48 (D1): D344-D353

2019

Protein Abundance Biases the Amino Acid Composition of Disordered Regions to Minimize Non-functional Interactions.
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J Mol Biol, ; 431 (24): 4978-4992
Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast.
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Nat Commun, ; 10 (1): 2960
Geometric description of self-interaction potential in symmetric protein complexes.
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Sci Data, ; 6 (1): 64
Infinite Assembly of Folded Proteins in Evolution, Disease, and Engineering.
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Angew Chem Int Ed Engl, ; 58 (17): 5514-5531
Evolthon: A community endeavor to evolve lab evolution.
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PLoS Biol, ; 17 (3): e3000182
YeastRGB: comparing the abundance and localization of yeast proteins across cells and libraries.
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Nucleic Acids Res, ; 47 (D1): D1245-D1249

2018

Genome-wide C-SWAT library for high-throughput yeast genome tagging.
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Nat Methods, ; 15 (8): 598-600
Genome-wide SWAp-Tag yeast libraries for proteome exploration.
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Nat Methods, ; 15 (8): 617-622
PDB-wide identification of biological assemblies from conserved quaternary structure geometry.
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Nat Methods, ; 15 (1): 67-72
Inferring and Using Protein Quaternary Structure Information from Crystallographic Data.
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Methods Mol Biol, ; 1764 : 357-375

2017

Proteins evolve on the edge of supramolecular self-assembly.
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Nature, ; 548 (7666): 244-247
Exhaustive search of linear information encoding protein-peptide recognition.
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PLoS Comput Biol, ; 13 (4): e1005499

2016

The Dihydrofolate Reductase Protein-Fragment Complementation Assay: A Survival-Selection Assay for Large-Scale Analysis of Protein-Protein Interactions.
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Cold Spring Harb Protoc, ; 2016 (11)
Protein-Fragment Complementation Assays for Large-Scale Analysis, Functional Dissection, and Spatiotemporal Dynamic Studies of Protein-Protein Interactions in Living Cells.
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Cold Spring Harb Protoc, ; 2016 (11)
Young phosphorylation is functionally silent.
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Science, ; 354 (6309): 176-177
Evolution of domain-peptide interactions to coadapt specificity and affinity to functional diversity.
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Proc Natl Acad Sci U S A, ; 113 (27): E3862-E3871

2015

Decoding a neural circuit controlling global animal state in C. elegans.
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Elife, ; 4
Symmetry breaking in homo-oligomers: the curious case of mega-hemocyanin.
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Structure, ; 23 (1): 3-5

2014

Fast and accurate discovery of degenerate linear motifs in protein sequences.
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PLoS One, ; 9 (9): e106081
Different subunits belonging to the same protein complex often exhibit discordant expression levels and evolutionary properties.
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Curr Opin Struct Biol, ; 26 : 113-120
High-resolution mapping of protein concentration reveals principles of proteome architecture and adaptation.
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Cell Rep, ; 7 (4): 1333-1340

2013

Extracting insight from noisy cellular networks.
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Cell, ; 155 (5): 983-989
Structural, evolutionary, and assembly principles of protein oligomerization.
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Prog Mol Biol Transl Sci, ; 117 : 25-51

2012

Cellular crowding imposes global constraints on the chemistry and evolution of proteomes.
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Proc Natl Acad Sci U S A, ; 109 (50): 20461-20466
Protein abundance is key to distinguish promiscuous from functional phosphorylation based on evolutionary information.
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Philos Trans R Soc Lond B Biol Sci, ; 367 (1602): 2594-2606

2010

A simple definition of structural regions in proteins and its use in analyzing interface evolution.
J Mol Biol, ; 403 (4): 660-670
Molecular characterization of the evolution of phagosomes.
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Mol Syst Biol, ; 6 : 423
Homomeric protein complexes: evolution and assembly.
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Biochem Soc Trans, ; 38 (4): 879-882
Systemizing the structures and structuring the system.
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Expert Rev Proteomics, ; 7 (3): 319-322
Cell signaling. Signaling through cooperation.
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Science, ; 328 (5981): 983-984

2009

Physicochemical principles that regulate the competition between functional and dysfunctional association of proteins.
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Proc Natl Acad Sci U S A, ; 106 (25): 10159-10164
Weak functional constraints on phosphoproteomes.
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Trends Genet, ; 25 (5): 193-197
Self-assembly and evolution of homomeric protein complexes.
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Phys Rev Lett, ; 102 (11): 118106
How perfect can protein interactomes be?
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Sci Signal, ; 2 (60): pe11

2008

Assembly reflects evolution of protein complexes.
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Nature, ; 453 (7199): 1262-1265
Evolution and dynamics of protein interactions and networks.
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Curr Opin Struct Biol, ; 18 (3): 349-357

2007

PiQSi: protein quaternary structure investigation.
Structure, ; 15 (11): 1364-1367
CORRIE: enzyme sequence annotation with confidence estimates.
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BMC Bioinformatics, ; 8 Suppl 4 (Suppl 4): S3
Evolution of protein complexes by duplication of homomeric interactions.
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Genome Biol, ; 8 (4): R51

2006

3D complex: a structural classification of protein complexes.
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PLoS Comput Biol, ; 2 (11): e155
The origins and evolution of functional modules: lessons from protein complexes.
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Philos Trans R Soc Lond B Biol Sci, ; 361 (1467): 507-517

2005

Probabilistic annotation of protein sequences based on functional classifications.
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BMC Bioinformatics, ; 6 : 302