Publications
2025
A feedback circuitry involving γ-actin, β-actin and nonmuscle myosin-2 A controls tight junction and apical cortex mechanics.
2024
Evidence for an interaction of paracingulin with microtubules.
Cingulin-nonmuscle myosin interaction plays a role in epithelial morphogenesis and cingulin nanoscale organization.
A short guide to the tight junction.
2023
Knock Out of CGN and CGNL1 in MDCK Cells Affects Claudin-2 but Has a Minor Impact on Tight Junction Barrier Function.
Cingulin and paracingulin tether myosins-2 to junctions to mechanoregulate the plasma membrane.
Paracingulin recruits CAMSAP3 to tight junctions and regulates microtubule and polarized epithelial cell organization.
2022
The PLEKHA7-PDZD11 complex regulates the localization of the calcium pump PMCA and calcium handling in cultured cells.
Cingulin binds to the ZU5 domain of scaffolding protein ZO-1 to promote its extended conformation, stabilization, and tight junction accumulation.
The ACE2 Receptor for Coronavirus Entry Is Localized at Apical Cell-Cell Junctions of Epithelial Cells.
2021
PLEKHA5, PLEKHA6, and PLEKHA7 bind to PDZD11 to target the Menkes ATPase ATP7A to the cell periphery and regulate copper homeostasis.
WW, PH and C-Terminal Domains Cooperate to Direct the Subcellular Localizations of PLEKHA5, PLEKHA6 and PLEKHA7.
2020
The tight junction protein cingulin regulates the vascular response to burn injury in a mouse model.
Scaffolding proteins of vertebrate apical junctions: structure, functions and biophysics.
Cooperative binding of the tandem WW domains of PLEKHA7 to PDZD11 promotes conformation-dependent interaction with tetraspanin 33.
Cell Biology: Tight Junctions as Biomolecular Condensates.
2019
The mechanobiology of tight junctions.
LncRNA EPR controls epithelial proliferation by coordinating Cdkn1a transcription and mRNA decay response to TGF-β.
R40.76 binds to the α domain of ZO-1: role of ZO-1 (α+) in epithelial differentiation and mechano-sensing.
2018
A Dock-and-Lock Mechanism Clusters ADAM10 at Cell-Cell Junctions to Promote α-Toxin Cytotoxicity.
Intestinal barriers protect against disease.
The role of microtubules in the regulation of epithelial junctions.
2017
Tension-Dependent Stretching Activates ZO-1 to Control the Junctional Localization of Its Interactors.
Cell-specific diversity in the expression and organization of cytoplasmic plaque proteins of apical junctions.
The role of apical cell-cell junctions and associated cytoskeleton in mechanotransduction.
2016
Role of Cingulin in Agonist-induced Vascular Endothelial Permeability.
Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells.
PLEKHA7: Cytoskeletal adaptor protein at center stage in junctional organization and signaling.
PLEKHA7 Recruits PDZD11 to Adherens Junctions to Stabilize Nectins.
Grete Kellenberger-Gujer: Molecular biology research pioneer.
Evidence That Cingulin Regulates Endothelial Barrier Function In Vitro and In Vivo.
2015
The adherens junctions control susceptibility to Staphylococcus aureus α-toxin.
Distinct E-cadherin-based complexes regulate cell behaviour through miRNA processing or Src and p120 catenin activity.
The Expression of the Zonula Adhaerens Protein PLEKHA7 Is Strongly Decreased in High Grade Ductal and Lobular Breast Carcinomas.
2014
ZO proteins redundantly regulate the transcription factor DbpA/ZONAB.
MgcRacGAP interacts with cingulin and paracingulin to regulate Rac1 activation and development of the tight junction barrier during epithelial junction assembly.
Toll-like receptor 2 regulates the barrier function of human bronchial epithelial monolayers through atypical protein kinase C zeta, and an increase in expression of claudin-1.
PLEKHA7 modulates epithelial tight junction barrier function.
Epithelial junctions and Rho family GTPases: the zonular signalosome.
2013
The junctional proteins cingulin and paracingulin modulate the expression of tight junction protein genes through GATA-4.
2012
Cingulin is dispensable for epithelial barrier function and tight junction structure, and plays a role in the control of claudin-2 expression and response to duodenal mucosa injury.
Cingulin, paracingulin, and PLEKHA7: signaling and cytoskeletal adaptors at the apical junctional complex.
Distinct domains of paracingulin are involved in its targeting to the actin cytoskeleton and regulation of apical junction assembly.
The control of gene expression and cell proliferation by the epithelial apical junctional complex.
2011
Regulation of small GTPases at epithelial cell-cell junctions.
A role for ZO-1 and PLEKHA7 in recruiting paracingulin to tight and adherens junctions of epithelial cells.
Cingulin and paracingulin show similar dynamic behaviour, but are recruited independently to junctions.
2010
PLEKHA7 is an adherens junction protein with a tissue distribution and subcellular localization distinct from ZO-1 and E-cadherin.
2009
The tight junction protein cingulin regulates gene expression and RhoA signaling.
2008
Paracingulin regulates the activity of Rac1 and RhoA GTPases by recruiting Tiam1 and GEF-H1 to epithelial junctions.
The cytoplasmic plaque of tight junctions: a scaffolding and signalling center.
Inducible overexpression of cingulin in stably transfected MDCK cells does not affect tight junction organization and gene expression.
2007
Tight junction formation in early Xenopus laevis embryos: identification and ultrastructural characterization of junctional crests and junctional vesicles.
Claudin-1 and claudin-5 expression patterns differentiate lung squamous cell carcinomas from adenocarcinomas.
2006
Cingulin regulates claudin-2 expression and cell proliferation through the small GTPase RhoA.
2005
Binding of GEF-H1 to the tight junction-associated adaptor cingulin results in inhibition of Rho signaling and G1/S phase transition.
2004
Histone deacetylase inhibitors up-regulate the expression of tight junction proteins.
Mol Cancer Res,
2004 Dec;
2
(12): 692-701
Disruption of the cingulin gene does not prevent tight junction formation but alters gene expression.
2003
What's new in tight junctions: a report on the 2002 ASCB Meeting, San Francisco, CA.
2002
Evidence for a functional interaction between cingulin and ZO-1 in cultured cells.
Molecular complexity of vertebrate tight junctions (Review).
2001
Cingulin interacts with F-actin in vitro.
2000
Differentiation of the epithelial apical junctional complex during mouse preimplantation development: a role for rab13 in the early maturation of the tight junction.
Tight junction biogenesis in the early Xenopus embryo.
Introduction: opening up tight junctions.
Human and Xenopus cingulin share a modular organization of the coiled-coil rod domain: predictions for intra- and intermolecular assembly.
Interaction of junctional adhesion molecule with the tight junction components ZO-1, cingulin, and occludin.
1999
Cingulin contains globular and coiled-coil domains and interacts with ZO-1, ZO-2, ZO-3, and myosin.
Xenopus laevis occludin. Identification of in vitro phosphorylation sites by protein kinase CK2 and association with cingulin.
Experimental colitis increases small intestine permeability in the rat.
Lab Invest,
1999 Jan;
79
(1): 49-57
1998
Tight junction proteins.
1997
Hepatocyte tight-junctional permeability is increased in rat experimental colitis.
1996
Tight junctions in early amphibian development: detection of junctional cingulin from the 2-cell stage and its localization at the boundary of distinct membrane domains in dividing blastomeres in low calcium.
Dev Dyn,
1996 Sep;
207
(1): 104-113
1995
Vascular smooth muscle cells of H-2Kb-tsA58 transgenic mice. Characterization of cell lines with distinct properties.
1994
Effect of protein kinase inhibitor H-7 on the contractility, integrity, and membrane anchorage of the microfilament system.
1993
The molecular organization of tight junctions.
Localisation of tight junction protein cingulin is temporally and spatially regulated during early mouse development.
Tight junction protein cingulin is expressed by maternal and embryonic genomes during early mouse development.
1992
Polarized functions and permeability properties of rat epididymal epithelial cells in vitro.
Protein kinase inhibitors prevent junction dissociation induced by low extracellular calcium in MDCK epithelial cells.
1991
The role of phosphorylation in development of tight junctions in cultured renal epithelial (MDCK) cells.
Cingulin, a specific protein component of tight junctions, is expressed in normal and neoplastic human epithelial tissues.
Am J Pathol,
1991 Apr;
138
(4): 781-789
Localization of myosin in the cytoskeleton of brush border cells using monoclonal antibodies and confocal laser-beam scanning microscopy.
1989
ZO-1 and cingulin: tight junction proteins with distinct identities and localizations.
Parallel modulation of brush border myosin conformation and enzyme activity induced by monoclonal antibodies.
Cingulin: characterization and localization.
1988
Modulation of cellular morphology and locomotory activity by antibodies against myosin.
Active site trapping of nucleotide by smooth and non-muscle myosins.
Brush border myosin filament assembly and interaction with actin investigated with monoclonal antibodies.
How phosphorylation controls the self-assembly of vertebrate smooth and non-muscle myosins.
Cingulin, a new peripheral component of tight junctions.
1987
Effects of light chain phosphorylation and skeletal myosin on the stability of non-muscle myosin filaments.
Polymerization of vertebrate non-muscle and smooth muscle myosins.
Regulation of non-muscle myosin structure and function.
Studies on the structure and conformation of brush border myosin using monoclonal antibodies.
1986
Regulation in vitro of brush border myosin by light chain phosphorylation.