Publications

2023

Essential roles of RNA cap-proximal ribose methylation in mammalian embryonic development and fertility.

Dohnalkova M, Krasnykov K, Mendel M, Li L, Panasenko O, Fleury-Olela F, Vågbø CB, Homolka D, Pillai RS

Cell Rep,2023 Jul 2542(7):

DOI: 10.1016/j.celrep.2023.112786

The catalytic-dead Pcif1 regulates gene expression and fertility in Drosophila.

Franco G, Taillebourg E, Delfino E, Homolka D, Gueguen N, Brasset E, Pandey RR, Pillai RS, Fauvarque MO

RNA,2023 May29(5):

DOI: 10.1261/rna.079192.122

Intermitochondrial cement (IMC) harbors piRNA biogenesis machinery and exonuclease domain-containing proteins EXD1 and EXD2 in mouse spermatocytes.

Olotu O, Dowling M, Homolka D, Wojtas MN, Tran P, Lehtiniemi T, Da Ros M, Pillai RS, Kotaja N

Andrology,2023 May11(4):

DOI: 10.1111/andr.13361

In vivo PIWI slicing in mouse testes deviates from rules established in vitro.

Dowling M, Homolka D, Raad N, Gos P, Pandey RR, Pillai RS

RNA,2023 Mar29(3):

DOI: 10.1261/rna.079349.122

2022

The XRN1-regulated RNA helicase activity of YTHDC2 ensures mouse fertility independently of m(6)A recognition.

Li L, Krasnykov K, Homolka D, Gos P, Mendel M, Fish RJ, Pandey RR, Pillai RS

Mol Cell,2022 May 582(9):

DOI: 10.1016/j.molcel.2022.02.034

2021

YTHDC2 is essential for pachytene progression and prevents aberrant microtubule-driven telomere clustering in male meiosis.

Liu R, Kasowitz SD, Homolka D, Leu NA, Shaked JT, Ruthel G, Jain D, Lin H, Keeney S, Luo M, Pillai RS, Wang PJ

Cell Rep,2021 Dec 1437(11):

DOI: 10.1016/j.celrep.2021.110110

Splice site m⁶A methylation prevents binding of U2AF35 to inhibit RNA splicing.

Mendel M, Delaney K, Pandey RR, Chen KM, Wenda JM, Vågbø CB, Steiner FA, Homolka D, Pillai RS

Cell,2021 Jun 10184(12):

DOI: 10.1016/j.cell.2021.03.062

2020

The Mammalian Cap-Specific m⁶Am RNA Methyltransferase PCIF1 Regulates Transcript Levels in Mouse Tissues.

Pandey RR, Delfino E, Homolka D, Roithova A, Chen KM, Li L, Franco G, Vågbø CB, Taillebourg E, Fauvarque MO, Pillai RS

Cell Rep,2020 Aug 1832(7):

DOI: 10.1016/j.celrep.2020.108038

TEX15 associates with MILI and silences transposable elements in male germ cells.

Yang F, Lan Y, Pandey RR, Homolka D, Berger SL, Pillai RS, Bartolomei MS, Wang PJ

Genes Dev,2020 Jun 134(11-12):

DOI: 10.1101/gad.335489.119

2019

Decapping Enzyme NUDT12 Partners with BLMH for Cytoplasmic Surveillance of NAD-Capped RNAs.

Wu H, Li L, Chen KM, Homolka D, Gos P, Fleury-Olela F, McCarthy AA, Pillai RS

Cell Rep,2019 Dec 2429(13):

DOI: 10.1016/j.celrep.2019.11.108

An RNA exporter that enforces a no-export policy.

Homolka D, Pillai RS

Nat Struct Mol Biol,2019 Sep26(9):

DOI: 10.1038/s41594-019-0294-y

Nxf3: a middleman with the right connections for unspliced piRNA precursor export.

Mendel M, Pillai RS

Genes Dev,2019 Sep 133(17-18):

DOI: 10.1101/gad.330530.119

Counting the Cuts: MAZTER-Seq Quantifies m⁶A Levels Using a Methylation-Sensitive Ribonuclease.

Pandey RR, Pillai RS

Cell,2019 Jul 25178(3):

DOI: 10.1016/j.cell.2019.07.006

2018

Methylation of Structured RNA by the m⁶A Writer METTL16 Is Essential for Mouse Embryonic Development.

Mendel M, Chen KM, Homolka D, Gos P, Pandey RR, McCarthy AA, Pillai RS

Mol Cell,2018 Sep 2071(6):

DOI: 10.1016/j.molcel.2018.08.004

Exonuclease Domain-Containing 1 Enhances MIWI2 piRNA Biogenesis via Its Interaction with TDRD12.

Pandey RR, Homolka D, Olotu O, Sachidanandam R, Kotaja N, Pillai RS

Cell Rep,2018 Sep 2524(13):

DOI: 10.1016/j.celrep.2018.08.087

2017

Regulation of m⁶A Transcripts by the 3'→5' RNA Helicase YTHDC2 Is Essential for a Successful Meiotic Program in the Mammalian Germline.

Wojtas MN, Pandey RR, Mendel M, Homolka D, Sachidanandam R, Pillai RS

Mol Cell,2017 Oct 1968(2):

DOI: 10.1016/j.molcel.2017.09.021

Selective termination of lncRNA transcription promotes heterochromatin silencing and cell differentiation.

Touat-Todeschini L, Shichino Y, Dangin M, Thierry-Mieg N, Gilquin B, Hiriart E, Sachidanandam R, Lambert E, Brettschneider J, Reuter M, Kadlec J, Pillai R, Yamashita A, Yamamoto M, Verdel A

EMBO J,2017 Sep 136(17):

DOI: 10.15252/embj.201796571

Recruitment of Armitage and Yb to a transcript triggers its phased processing into primary piRNAs in Drosophila ovaries.

Pandey RR, Homolka D, Chen KM, Sachidanandam R, Fauvarque MO, Pillai RS

PLoS Genet,2017 Aug13(8):

DOI: 10.1371/journal.pgen.1006956

Distinct Roles of RNA Helicases MVH and TDRD9 in PIWI Slicing-Triggered Mammalian piRNA Biogenesis and Function.

Wenda JM, Homolka D, Yang Z, Spinelli P, Sachidanandam R, Pandey RR, Pillai RS

Dev Cell,2017 Jun 1941(6):

DOI: 10.1016/j.devcel.2017.05.021

Characterization of the mammalian RNA exonuclease 5/NEF-sp as a testis-specific nuclear 3' → 5' exoribonuclease.

Silva S, Homolka D, Pillai RS

RNA,2017 Sep23(9):

DOI: 10.1261/rna.060723.117

2016

Mutations in the MOV10L1 ATP Hydrolysis Motif Cause piRNA Biogenesis Failure and Male Sterility in Mice.

Fu Q, Pandey RR, Leu NA, Pillai RS, Wang PJ

Biol Reprod,2016 Nov 195(5):

DOI: 10.1095/biolreprod.116.142430

PIWI Slicing and EXD1 Drive Biogenesis of Nuclear piRNAs from Cytosolic Targets of the Mouse piRNA Pathway.

Yang Z, Chen KM, Pandey RR, Homolka D, Reuter M, Janeiro BK, Sachidanandam R, Fauvarque MO, McCarthy AA, Pillai RS

Mol Cell,2016 Jan 761(1):

DOI: 10.1016/j.molcel.2015.11.009

2015

PIWI Slicing and RNA Elements in Precursors Instruct Directional Primary piRNA Biogenesis.

Homolka D, Pandey RR, Goriaux C, Brasset E, Vaury C, Sachidanandam R, Fauvarque MO, Pillai RS

Cell Rep,2015 Jul 2112(3):

DOI: 10.1016/j.celrep.2015.06.030

Metazoan Maelstrom is an RNA-binding protein that has evolved from an ancient nuclease active in protists.

Chen KM, Campbell E, Pandey RR, Yang Z, McCarthy AA, Pillai RS

RNA,2015 May21(5):

DOI: 10.1261/rna.049437.114

The RNA helicase MOV10L1 binds piRNA precursors to initiate piRNA processing.

Vourekas A, Zheng K, Fu Q, Maragkakis M, Alexiou P, Ma J, Pillai RS, Mourelatos Z, Wang PJ

Genes Dev,2015 Mar 1529(6):

DOI: 10.1101/gad.254631.114

2014

Fly piRNA biogenesis: tap dancing with Tej.

Yang Z, Pillai RS

BMC Biol,2014 Oct 612:

DOI: 10.1186/s12915-014-0077-1

Primary piRNA biogenesis: caught up in a Maelstrom.

Pandey RR, Pillai RS

EMBO J,2014 Sep 1733(18):

DOI: 10.15252/embj.201489670

RNA clamping by Vasa assembles a piRNA amplifier complex on transposon transcripts.

Xiol J, Spinelli P, Laussmann MA, Homolka D, Yang Z, Cora E, Couté Y, Conn S, Kadlec J, Sachidanandam R, Kaksonen M, Cusack S, Ephrussi A, Pillai RS

Cell,2014 Jun 19157(7):

DOI: 10.1016/j.cell.2014.05.018

The MID-PIWI module of Piwi proteins specifies nucleotide- and strand-biases of piRNAs.

Cora E, Pandey RR, Xiol J, Taylor J, Sachidanandam R, McCarthy AA, Pillai RS

RNA,2014 Jun20(6):

DOI: 10.1261/rna.044701.114

Impact of nuclear Piwi elimination on chromatin state in Drosophila melanogaster ovaries.

Klenov MS, Lavrov SA, Korbut AP, Stolyarenko AD, Yakushev EY, Reuter M, Pillai RS, Gvozdev VA

Nucleic Acids Res,2014 Jun42(10):

DOI: 10.1093/nar/gku268

Analysis of small RNA-guided endonuclease activity in endogenous Piwi protein complexes from mouse testes.

Reuter M, Pillai RS

Methods Mol Biol,20141093:

DOI: 10.1007/978-1-62703-694-8_9

2013

Tudor domain containing 12 (TDRD12) is essential for secondary PIWI interacting RNA biogenesis in mice.

Pandey RR, Tokuzawa Y, Yang Z, Hayashi E, Ichisaka T, Kajita S, Asano Y, Kunieda T, Sachidanandam R, Chuma S, Yamanaka S, Pillai RS

Proc Natl Acad Sci U S A,2013 Oct 8110(41):

DOI: 10.1073/pnas.1316316110

[The piRNAs forge an immune system for the genome].

Muller S, Raman Pandey R, Pillai RS

Med Sci (Paris),2013 May29(5):

DOI: 10.1051/medsci/2013295011

2012

Crystal structure of the primary piRNA biogenesis factor Zucchini reveals similarity to the bacterial PLD endonuclease Nuc.

Voigt F, Reuter M, Kasaruho A, Schulz EC, Pillai RS, Barabas O

RNA,2012 Dec18(12):

DOI: 10.1261/rna.034967.112

The multiple Tudor domain-containing protein TDRD1 is a molecular scaffold for mouse Piwi proteins and piRNA biogenesis factors.

Mathioudakis N, Palencia A, Kadlec J, Round A, Tripsianes K, Sattler M, Pillai RS, Cusack S

RNA,2012 Nov18(11):

DOI: 10.1261/rna.034181.112

A role for Fkbp6 and the chaperone machinery in piRNA amplification and transposon silencing.

Xiol J, Cora E, Koglgruber R, Chuma S, Subramanian S, Hosokawa M, Reuter M, Yang Z, Berninger P, Palencia A, Benes V, Penninger J, Sachidanandam R, Pillai RS

Mol Cell,2012 Sep 2847(6):

DOI: 10.1016/j.molcel.2012.07.019

Molecular biology. Outsourcing genome protection.

Xiol J, Pillai RS

Science,2012 Aug 3337(6094):

DOI: 10.1126/science.1227095

piRNAs and their involvement in male germline development in mice.

Pillai RS, Chuma S

Dev Growth Differ,2012 Jan54(1):

DOI: 10.1111/j.1440-169X.2011.01320.x

2011

Miwi catalysis is required for piRNA amplification-independent LINE1 transposon silencing.

Reuter M, Berninger P, Chuma S, Shah H, Hosokawa M, Funaya C, Antony C, Sachidanandam R, Pillai RS

Nature,2011 Nov 27480(7376):

DOI: 10.1038/nature10672

Tudor domain containing 7 (Tdrd7) is essential for dynamic ribonucleoprotein (RNP) remodeling of chromatoid bodies during spermatogenesis.

Tanaka T, Hosokawa M, Vagin VV, Reuter M, Hayashi E, Mochizuki AL, Kitamura K, Yamanaka H, Kondoh G, Okawa K, Kuramochi-Miyagawa S, Nakano T, Sachidanandam R, Hannon GJ, Pillai RS, Nakatsuji N, Chuma S

Proc Natl Acad Sci U S A,2011 Jun 28108(26):

DOI: 10.1073/pnas.1015447108

Recognition of 2'-O-methylated 3'-end of piRNA by the PAZ domain of a Piwi protein.

Simon B, Kirkpatrick JP, Eckhardt S, Reuter M, Rocha EA, Andrade-Navarro MA, Sehr P, Pillai RS, Carlomagno T

Structure,2011 Feb 919(2):

DOI: 10.1016/j.str.2010.11.015

2010

Mouse MOV10L1 associates with Piwi proteins and is an essential component of the Piwi-interacting RNA (piRNA) pathway.

Zheng K, Xiol J, Reuter M, Eckardt S, Leu NA, McLaughlin KJ, Stark A, Sachidanandam R, Pillai RS, Wang PJ

Proc Natl Acad Sci U S A,2010 Jun 29107(26):

DOI: 10.1073/pnas.1003953107

2009

Retrotransposon silencing by piRNAs: ping-pong players mark their sub-cellular boundaries.

Chuma S, Pillai RS

PLoS Genet,2009 Dec5(12):

DOI: 10.1371/journal.pgen.1000770

The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline.

Shoji M, Tanaka T, Hosokawa M, Reuter M, Stark A, Kato Y, Kondoh G, Okawa K, Chujo T, Suzuki T, Hata K, Martin SL, Noce T, Kuramochi-Miyagawa S, Nakano T, Sasaki H, Pillai RS, Nakatsuji N, Chuma S

Dev Cell,2009 Dec17(6):

DOI: 10.1016/j.devcel.2009.10.012

Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile.

Reuter M, Chuma S, Tanaka T, Franz T, Stark A, Pillai RS

Nat Struct Mol Biol,2009 Jun16(6):

DOI: 10.1038/nsmb.1615

2007

MicroRNA inhibition of translation initiation in vitro by targeting the cap-binding complex eIF4F.

Mathonnet G, Fabian MR, Svitkin YV, Parsyan A, Huck L, Murata T, Biffo S, Merrick WC, Darzynkiewicz E, Pillai RS, Filipowicz W, Duchaine TF, Sonenberg N

Science,2007 Sep 21317(5845):

DOI: 10.1126/science.1146067

Repression of protein synthesis by miRNAs: how many mechanisms?

Pillai RS, Bhattacharyya SN, Filipowicz W

Trends Cell Biol,2007 Mar17(3):

DOI: 10.1016/j.tcb.2006.12.007

2006

Effects of Dicer and Argonaute down-regulation on mRNA levels in human HEK293 cells.

Schmitter D, Filkowski J, Sewer A, Pillai RS, Oakeley EJ, Zavolan M, Svoboda P, Filipowicz W

Nucleic Acids Res,200634(17):

DOI: 10.1093/nar/gkl646

2005

MicroRNA function: multiple mechanisms for a tiny RNA?

Pillai RS

RNA,2005 Dec11(12):

DOI: 10.1261/rna.2248605

Toward an assembly line for U7 snRNPs: interactions of U7-specific Lsm proteins with PRMT5 and SMN complexes.

Azzouz TN, Pillai RS, Däpp C, Chari A, Meister G, Kambach C, Fischer U, Schümperli D

J Biol Chem,2005 Oct 14280(41):

DOI: 10.1074/jbc.M505077200

Inhibition of translational initiation by Let-7 MicroRNA in human cells.

Pillai RS, Bhattacharyya SN, Artus CG, Zoller T, Cougot N, Basyuk E, Bertrand E, Filipowicz W

Science,2005 Sep 2309(5740):

DOI: 10.1126/science.1115079

The C-terminal domain of coilin interacts with Sm proteins and U snRNPs.

Xu H, Pillai RS, Azzouz TN, Shpargel KB, Kambach C, Hebert MD, Schümperli D, Matera AG

Chromosoma,2005 Aug114(3):

DOI: 10.1007/s00412-005-0003-y

Post-transcriptional gene silencing by siRNAs and miRNAs.

Filipowicz W, Jaskiewicz L, Kolb FA, Pillai RS

Curr Opin Struct Biol,2005 Jun15(3):

DOI: 10.1016/j.sbi.2005.05.006

2004

Tethering of human Ago proteins to mRNA mimics the miRNA-mediated repression of protein synthesis.

Pillai RS, Artus CG, Filipowicz W

RNA,2004 Oct10(10):

DOI: 10.1261/rna.7131604

The special Sm core structure of the U7 snRNP: far-reaching significance of a small nuclear ribonucleoprotein.

Schümperli D, Pillai RS

Cell Mol Life Sci,2004 Oct61(19-20):

DOI: 10.1007/s00018-004-4190-0

2003

Unique Sm core structure of U7 snRNPs: assembly by a specialized SMN complex and the role of a new component, Lsm11, in histone RNA processing.

Pillai RS, Grimmler M, Meister G, Will CL, Lührmann R, Fischer U, Schümperli D

Genes Dev,2003 Sep 1517(18):

DOI: 10.1101/gad.274403

2001

A multiprotein complex mediates the ATP-dependent assembly of spliceosomal U snRNPs.

Meister G, Bühler D, Pillai R, Lottspeich F, Fischer U

Nat Cell Biol,2001 Nov3(11):

DOI: 10.1038/ncb1101-945

Purified U7 snRNPs lack the Sm proteins D1 and D2 but contain Lsm10, a new 14 kDa Sm D1-like protein.

Pillai RS, Will CL, Lührmann R, Schümperli D, Müller B

EMBO J,2001 Oct 120(19):

DOI: 10.1093/emboj/20.19.5470

Ramesh Pillai