Publications

2023

First full views of a CRISPR-guided system for gene insertion.

Barabas O, Rice PA

Nature,2023 Jan 11:

2022

Landscape of mobile genetic elements and their antibiotic resistance cargo in prokaryotic genomes.

Khedkar S, Smyshlyaev G, Letunic I, Maistrenko OM, Coelho LP, Orakov A, Forslund SK, Hildebrand F, Luetge M, Schmidt TSB, Barabas O, Bork P

Nucleic Acids Res,2022 Apr 850(6):

DOI: 10.1093/nar/gkac163

Engineered Sleeping Beauty transposase redirects transposon integration away from genes.

Miskey C, Kesselring L, Querques I, Abrusán G, Barabas O, Ivics Z

Nucleic Acids Res,2022 Mar 2150(5):

DOI: 10.1093/nar/gkac092

2021

Sequence analysis of tyrosine recombinases allows annotation of mobile genetic elements in prokaryotic genomes.

Smyshlyaev G, Bateman A, Barabas O

Mol Syst Biol,2021 May17(5):

DOI: 10.15252/msb.20209880

Towards Dissecting the Mechanism of Protein Phosphatase-1 Inhibition by Its C-Terminal Phosphorylation.

Salvi F, Hoermann B, Del Pino García J, Fontanillo M, Derua R, Beullens M, Bollen M, Barabas O, Köhn M

Chembiochem,2021 Mar 222(5):

DOI: 10.1002/cbic.202000669

2020

A single amino acid switch converts the Sleeping Beauty transposase into an efficient unidirectional excisionase with utility in stem cell reprogramming.

Kesselring L, Miskey C, Zuliani C, Querques I, Kapitonov V, Laukó A, Fehér A, Palazzo A, Diem T, Lustig J, Sebe A, Wang Y, Dinnyés A, Izsvák Z, Barabas O, Ivics Z

Nucleic Acids Res,2020 Jan 1048(1):

DOI: 10.1093/nar/gkz1119

2019

A highly soluble Sleeping Beauty transposase improves control of gene insertion.

Querques I, Mades A, Zuliani C, Miskey C, Alb M, Grueso E, Machwirth M, Rausch T, Einsele H, Ivics Z, Hudecek M, Barabas O

Nat Biotechnol,2019 Dec37(12):

DOI: 10.1038/s41587-019-0291-z

Jump ahead with a twist: DNA acrobatics drive transposition forward.

Arinkin V, Smyshlyaev G, Barabas O

Curr Opin Struct Biol,2019 Dec59:

DOI: 10.1016/j.sbi.2019.08.006

Snapshots of a genetic cut-and-paste.

Barabas O

Nature,2019 Nov575(7783):

DOI: 10.1038/d41586-019-03428-0

The USTC co-opts an ancient machinery to drive piRNA transcription in C. elegans.

Weng C, Kosalka J, Berkyurek AC, Stempor P, Feng X, Mao H, Zeng C, Li WJ, Yan YH, Dong MQ, Morero NR, Zuliani C, Barabas O, Ahringer J, Guang S, Miska EA

Genes Dev,2019 Jan 133(1-2):

DOI: 10.1101/gad.319293.118

2018

Effects of stably incorporated iron on protein phosphatase-1 structure and activity.

Salvi F, Trebacz M, Kokot T, Hoermann B, Rios P, Barabas O, Köhn M

FEBS Lett,2018 Dec592(24):

DOI: 10.1002/1873-3468.13284

Targeting IS608 transposon integration to highly specific sequences by structure-based transposon engineering.

Morero NR, Zuliani C, Kumar B, Bebel A, Okamoto S, Guynet C, Hickman AB, Chandler M, Dyda F, Barabas O

Nucleic Acids Res,2018 May 446(8):

DOI: 10.1093/nar/gky235

Transposase-DNA Complex Structures Reveal Mechanisms for Conjugative Transposition of Antibiotic Resistance.

Rubio-Cosials A, Schulz EC, Lambertsen L, Smyshlyaev G, Rojas-Cordova C, Forslund K, Karaca E, Bebel A, Bork P, Barabas O

Cell,2018 Mar 22173(1):

DOI: 10.1016/j.cell.2018.02.032

Conjugative transposition of the vancomycin resistance carrying Tn1549: enzymatic requirements and target site preferences.

Lambertsen L, Rubio-Cosials A, Patil KR, Barabas O

Mol Microbiol,2018 Mar107(5):

DOI: 10.1111/mmi.13905

2017

Intermolecular base stacking mediates RNA-RNA interaction in a crystal structure of the RNA chaperone Hfq.

Schulz EC, Seiler M, Zuliani C, Voigt F, Rybin V, Pogenberg V, Mücke N, Wilmanns M, Gibson TJ, Barabas O

Sci Rep,2017 Aug 297(1):

DOI: 10.1038/s41598-017-10085-8

2016

Structural snapshots of Xer recombination reveal activation by synaptic complex remodeling and DNA bending.

Bebel A, Karaca E, Kumar B, Stark WM, Barabas O

Elife,2016 Dec 235:

DOI: 10.7554/eLife.19706

Structural Determinants of Sleeping Beauty Transposase Activity.

Abrusán G, Yant SR, Szilágyi A, Marsh JA, Mátés L, Izsvák Z, Barabás O, Ivics Z

Mol Ther,2016 Aug24(8):

DOI: 10.1038/mt.2016.110

Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering.

Voigt F, Wiedemann L, Zuliani C, Querques I, Sebe A, Mátés L, Izsvák Z, Ivics Z, Barabas O

Nat Commun,2016 Mar 307:

DOI: 10.1038/ncomms11126

2014

An integrated approach for genome annotation of the eukaryotic thermophile Chaetomium thermophilum.

Bock T, Chen WH, Ori A, Malik N, Silva-Martin N, Huerta-Cepas J, Powell ST, Kastritis PL, Smyshlyaev G, Vonkova I, Kirkpatrick J, Doerks T, Nesme L, Baßler J, Kos M, Hurt E, Carlomagno T, Gavin AC, Barabas O, Müller CW, van Noort V, Beck M, Bork P

Nucleic Acids Res,2014 Dec 1642(22):

DOI: 10.1093/nar/gku1147

Structure of an Escherichia coli Hfq:RNA complex at 0.97 Å resolution.

Schulz EC, Barabas O

Acta Crystallogr F Struct Biol Commun,2014 Nov70(Pt 11):

DOI: 10.1107/S2053230X14020044

2013

Acquisition of an Archaea-like ribonuclease H domain by plant L1 retrotransposons supports modular evolution.

Smyshlyaev G, Voigt F, Blinov A, Barabas O, Novikova O

Proc Natl Acad Sci U S A,2013 Dec 10110(50):

DOI: 10.1073/pnas.1310958110

Catalytic mechanism of α-phosphate attack in dUTPase is revealed by X-ray crystallographic snapshots of distinct intermediates, 31P-NMR spectroscopy and reaction path modelling.

Barabás O, Németh V, Bodor A, Perczel A, Rosta E, Kele Z, Zagyva I, Szabadka Z, Grolmusz VI, Wilmanns M, Vértessy BG

Nucleic Acids Res,2013 Dec41(22):

DOI: 10.1093/nar/gkt756

2012

Crystal structure of the primary piRNA biogenesis factor Zucchini reveals similarity to the bacterial PLD endonuclease Nuc.

Voigt F, Reuter M, Kasaruho A, Schulz EC, Pillai RS, Barabas O

RNA,2012 Dec18(12):

DOI: 10.1261/rna.034967.112

2010

DNA recognition and the precleavage state during single-stranded DNA transposition in D. radiodurans.

Hickman AB, James JA, Barabas O, Pasternak C, Ton-Hoang B, Chandler M, Sommer S, Dyda F

EMBO J,2010 Nov 1729(22):

DOI: 10.1038/emboj.2010.241

2009

Helicobacter Pylori's plasticity zones are novel transposable elements.

Kersulyte D, Lee W, Subramaniam D, Anant S, Herrera P, Cabrera L, Balqui J, Barabas O, Kalia A, Gilman RH, Berg DE

PLoS One,2009 Sep 34(9):

DOI: 10.1371/journal.pone.0006859

Resetting the site: redirecting integration of an insertion sequence in a predictable way.

Guynet C, Achard A, Hoang BT, Barabas O, Hickman AB, Dyda F, Chandler M

Mol Cell,2009 Jun 1234(5):

DOI: 10.1016/j.molcel.2009.05.017

Molecular shape and prominent role of beta-strand swapping in organization of dUTPase oligomers.

Takács E, Barabás O, Petoukhov MV, Svergun DI, Vértessy BG

FEBS Lett,2009 Mar 4583(5):

DOI: 10.1016/j.febslet.2009.02.011

2008

Active site of mycobacterial dUTPase: structural characteristics and a built-in sensor.

Varga B, Barabás O, Takács E, Nagy N, Nagy P, Vértessy BG

Biochem Biophys Res Commun,2008 Aug 15373(1):

DOI: 10.1016/j.bbrc.2008.05.130

Methylene substitution at the alpha-beta bridging position within the phosphate chain of dUDP profoundly perturbs ligand accommodation into the dUTPase active site.

Kovári J, Barabás O, Varga B, Békési A, Tölgyesi F, Fidy J, Nagy J, Vértessy BG

Proteins,2008 Apr71(1):

DOI: 10.1002/prot.21757

Revisiting the mechanism of the autoactivation of the complement protease C1r in the C1 complex: structure of the active catalytic region of C1r.

Kardos J, Harmat V, Palló A, Barabás O, Szilágyi K, Gráf L, Náray-Szabó G, Goto Y, Závodszky P, Gál P

Mol Immunol,2008 Mar45(6):

DOI: 10.1016/j.molimm.2007.09.031

In vitro reconstitution of a single-stranded transposition mechanism of IS608.

Guynet C, Hickman AB, Barabas O, Dyda F, Chandler M, Ton-Hoang B

Mol Cell,2008 Feb 1529(3):

DOI: 10.1016/j.molcel.2007.12.008

Mechanism of IS200/IS605 family DNA transposases: activation and transposon-directed target site selection.

Barabas O, Ronning DR, Guynet C, Hickman AB, Ton-Hoang B, Chandler M, Dyda F

Cell,2008 Jan 25132(2):

DOI: 10.1016/j.cell.2007.12.029

2007

Active site closure facilitates juxtaposition of reactant atoms for initiation of catalysis by human dUTPase.

Varga B, Barabás O, Kovári J, Tóth J, Hunyadi-Gulyás E, Klement E, Medzihradszky KF, Tölgyesi F, Fidy J, Vértessy BG

FEBS Lett,2007 Oct 2581(24):

DOI: 10.1016/j.febslet.2007.09.005

Flexible segments modulate co-folding of dUTPase and nucleocapsid proteins.

Németh-Pongrácz V, Barabás O, Fuxreiter M, Simon I, Pichová I, Rumlová M, Zábranská H, Svergun D, Petoukhov M, Harmat V, Klement E, Hunyadi-Gulyás E, Medzihradszky KF, Kónya E, Vértessy BG

Nucleic Acids Res,200735(2):

DOI: 10.1093/nar/gkl1074

2006

Crystallization and preliminary X-ray studies of dUTPase from Mason-Pfizer monkey retrovirus.

Barabás O, Németh V, Vértessy BG

Acta Crystallogr Sect F Struct Biol Cryst Commun,2006 Apr 162(Pt 4):

DOI: 10.1107/S1744309106008931

2004

Structural insights into the catalytic mechanism of phosphate ester hydrolysis by dUTPase.

Barabás O, Pongrácz V, Kovári J, Wilmanns M, Vértessy BG

J Biol Chem,2004 Oct 8279(41):

DOI: 10.1074/jbc.M406135200

Altered active site flexibility and a structural metal-binding site in eukaryotic dUTPase: kinetic characterization, folding, and crystallographic studies of the homotrimeric Drosophila enzyme.

Kovári J, Barabás O, Takács E, Békési A, Dubrovay Z, Pongrácz V, Zagyva I, Imre T, Szabó P, Vértessy BG

J Biol Chem,2004 Apr 23279(17):

DOI: 10.1074/jbc.M313643200

2003

dUTPase and nucleocapsid polypeptides of the Mason-Pfizer monkey virus form a fusion protein in the virion with homotrimeric organization and low catalytic efficiency.

Barabás O, Rumlová M, Erdei A, Pongrácz V, Pichová I, Vértessy BG

J Biol Chem,2003 Oct 3278(40):

DOI: 10.1074/jbc.M306967200

2002

Synthesis and stereochemistry of dispiro substituted pyridazines: application of ellipticity-absorbance ratio spectra for proving enantiomeric relationship by HPLC-CD/UV detection.

Beke G, Gergely A, Szász G, Szentesi A, Nyitray J, Barabás O, Harmath V, Mátyus P

Chirality,2002 May 1514(5):

DOI: 10.1002/chir.10096

Orsolya Barabas